{"created":"2023-06-19T09:07:10.768019+00:00","id":585,"links":{},"metadata":{"_buckets":{"deposit":"34e69525-3219-497b-9f05-b325b6af01dd"},"_deposit":{"created_by":15,"id":"585","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"585"},"status":"published"},"_oai":{"id":"oai:stella.repo.nii.ac.jp:00000585","sets":[]},"author_link":["957","577","576","956","958"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"860","bibliographicPageStart":"856","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Previously, we prepared cationic nanoparticles (NP and NP-N) composed of cholesteryl diamine (OH-Chol, (3S)-N-(2-(2-hydroxyethylamino)ethyl)cholesteryl-3-carboxamide) and cholesteryl triamine (OH-N-Chol, (3S)-N-(2-(2-(2-hydroxyethylamino)ethylamino)ethyl)cholesteryl-3-carboxamide), respectively, with Tween 80 for small interfering RNA (siRNA) delivery into tumor cells. In this study, we prepared NP-0.25N composed of OH-Chol and OH-N-Chol at a molar ratio of 3/1 with Tween 80, and evaluated the transfection efficiency of plasmid DNA (pDNA) into tumor cells. NP-N exhibited lower transfection activity than NP; however, NP-0.25N showed higher transfection activity than both NP and NP-N in various tumor cells. NP-0.25N increased the amount of internalized pDNA by increased cellular association, and improved the escape from endosomes after clathrin-mediated endocytosis. The results of the experiments suggested that cholesteryl triamine may have potential as a helper lipid to increase the transfection for pDNA delivery by cationic cholesterol-based nanoparticles.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_10001_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"130003361415","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:10.1248/bpb.b12-01062","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本薬学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10885497","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0918-6158","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hattori, Yoshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"577","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090350222","nameIdentifierScheme":"NRID","nameIdentifierURI":" "},{"nameIdentifier":"90350222","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90350222"}]},{"creatorNames":[{"creatorName":"Nakamura, Tsukasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"956","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohno, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"957","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujii, Nobutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"958","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maitani, Yoshie","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"576","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010231581","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000010231581"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-08"}],"displaytype":"detail","filename":"BiolPharmBull_36‐5_p856.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BiolPharmBull_36‐5_p856.pdf ","url":"https://stella.repo.nii.ac.jp/record/585/files/BiolPharmBull_36‐5_p856.pdf"},"version_id":"cade7c02-a8fa-41a4-b72f-31e7f6edd446"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cationic nanoparticle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"plasmid DNA delivery","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transfection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cholesteryl diamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cholesteryl triamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Enhanced Plasmid DNA Transfer into Tumor Cells by Nanoparticle Composed of Cholesteryl Triamine and Diamine","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Enhanced Plasmid DNA Transfer into Tumor Cells by Nanoparticle Composed of Cholesteryl Triamine and Diamine","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["128"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-08"},"publish_date":"2016-02-08","publish_status":"0","recid":"585","relation_version_is_last":true,"title":["Enhanced Plasmid DNA Transfer into Tumor Cells by Nanoparticle Composed of Cholesteryl Triamine and Diamine"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-19T09:17:29.595176+00:00"}