{"created":"2023-06-19T09:07:10.510753+00:00","id":580,"links":{},"metadata":{"_buckets":{"deposit":"ca8e2ebd-55ea-4209-981f-06bf3d659c61"},"_deposit":{"created_by":15,"id":"580","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"580"},"status":"published"},"_oai":{"id":"oai:stella.repo.nii.ac.jp:00000580","sets":[]},"author_link":["464","675","130"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"702","bibliographicPageStart":"698","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Elastic fiber assembly is a complex stepwise process involving multiple different proteins and enzymes. Domain 36, encoded by the last exon of the elastin gene, is recognized to be an important domain for deposition onto microfibrils, an essential step in elastic fiber assembly. However, the role of domain 36 in elastic fiber assembly has not been clarified. Here, we utilized our established in vitro assembly model to identify the importance of domain 36 for the assembly process. Our results showed that the lack of domain 36 in bovine tropoelastin results in deficient elastic fiber assembly. A similar result was obtained with the point mutation of two cysteine residues and the deletion of the Lysine–Arginine–Lysine–Arginine (RKRK) sequence in domain 36. Double immunofluorescence of tropoelastin and fibrillin-1, a main component of microfibrils, demonstrated reduced localization of these mutant tropoelastin molecules on fibrillin-1 fibers. Moreover, the binding affinity of these mutants to fibrillin-1 and microfibril-associated glycoprotein (MAGP) was significantly decreased. These data indicate that domain 36 of tropoelastin facilitates elastic fiber assembly by interacting with microfibrils via two cysteine residues and the RKRK sequence.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_10001_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"130003390970","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:10.1248/bpb.b13-00933","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本薬学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10885497","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0918-6158","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nonaka, Risa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Fumiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"464","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010468580","nameIdentifierScheme":"NRID","nameIdentifierURI":" "},{"nameIdentifier":"10468580","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10468580"}]},{"creatorNames":[{"creatorName":"Wachi, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"130","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050318614","nameIdentifierScheme":"NRID","nameIdentifierURI":" "},{"nameIdentifier":"50318614","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50318614"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-08"}],"displaytype":"detail","filename":"BiolPharmBull_37‐4_p698.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BiolPharmBull_37‐4_p698.pdf ","url":"https://stella.repo.nii.ac.jp/record/580/files/BiolPharmBull_37‐4_p698.pdf"},"version_id":"cf51cb64-ef98-4ca1-a287-aef80c8719a5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"elastic fiber formation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tropoelastin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microfibril","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Domain 36 of Tropoelastin in Elastic Fiber Formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Domain 36 of Tropoelastin in Elastic Fiber Formation","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["128"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-08"},"publish_date":"2016-02-08","publish_status":"0","recid":"580","relation_version_is_last":true,"title":["Domain 36 of Tropoelastin in Elastic Fiber Formation"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-19T09:17:35.847990+00:00"}