@article{oai:stella.repo.nii.ac.jp:00000445,
 author = {Wachi, Hiroshi and Sugitani, Hideki and Tajima, Shingo and Seyama, Yoshiyuki},
 issue = {6},
 journal = {Journal of health science},
 month = {Dec},
 note = {application/pdf, Endothelin-1(ET-1) is known as a potent stimulator of cell proliferation and as a vasoconstrictor. It is believed that ET-1 contributes to the development of arterial diseases such as atherosclerosis. In this study, we demonstrated the expression of tropoelastin and lysyl oxidase(LO) on gene levels as induced by ET-1 in cultured smooth muscle cells(SMCs). ET-1 stimulated cell proliferation in a dose-dependent manner, and the level of this proliferation increased about 1.3-fold at 100 nM of ET-1. ET-1 suppressed the tropoelastin protein synthesis in a dose-dependent and time-dependent manner. In addition, ET-1 dose-dependently suppressed the tropoelastin and LO mRNA expression. The tropoelastin and LO mRNA levels decreased to about half and 4/5, respectively, at 100 nM of ET-1. The inhibition of elastin synthesis was completely prevented by BQ123, an endothelin receptor A(ET_A) antagonist. These results indicate that ET-1 can modulate the tropoelastin and LO mRNA expression via an ET_A receptor in cultured SMC and that the regulator for elastin expression may play an important role in elastogenesis and SMC proliferation during the development of atherosclerosis.},
 pages = {525--532},
 title = {Endothelin-1 Down-Regulates Expression of Tropoelastin and Lysyl Oxidase mRNA in Cultured Chick Aortic Smooth Muscle Cells},
 volume = {47},
 year = {2001}
}