@article{oai:stella.repo.nii.ac.jp:00000439,
 author = {Moriyama, Hiroyoshi and Hosoe, Tomoo and Wakana, Daigo and Itabashi, Takeshi and Kawai, Kenichi and Iizuka, Toru and Hoshi, Keiko and Fukushima, Kazutaka and Lau, Francis Chun},
 issue = {1},
 journal = {Journal of health science},
 month = {Feb},
 note = {application/pdf, A particle-counting aggregometer employing laser-light scattering was used in systematic manners to screen and to detect inhibitor(s) of platelet aggregation from the extract of Malbranchea filamentosa (M. filamentosa) IFM 41300. The inhibitor was determined as adenosine on the basis of the ^<13>C- and ^1H-NMR spectral data. Although adenosine was previously reported as an inhibitor of platelet aggregation, we isolated the compound from the fungus for the first time. Because the method was able to provide us with information on the developmental formation of platelet aggregates in different sizes with incubation time, we further elaborated the inhibitory behaviors of adenosine, as an example, at varied concentrations in different solvents such as dimethyl sulfoxide (DMSO) and saline. We found that DMSO could facilitate to dissolve less water soluble materials from herbs and fungi by using the present assay method.},
 pages = {103--108},
 title = {Assay-guided Informatory Screening Method for Antiplatelet Effect of Adenosine Isolated from Malbranchea filamentosa IFM 41300 : Inhibitory Behaviors of Adenosine in Different Solvents},
 volume = {55},
 year = {2009}
}